DNA In Situ Hybridization

DNAmRNA

HistoGeneX has been developing numerous FISH assays for exploratory and late phase clinical trials since 2003. In addition, we have deployed cytogenetic FISH assays for diagnosis, prognosis and therapeutic surveillance of cancer patients.

Use our team's experience to define pre-analytic variables and experimental conditions for your biomarker assay.

FISH assays are performed on an automated system with hybridization steps on calibrated heating modules. This semi-automated process can handle up to 50 slides in one run. SISH assays are performed on the automated system Benchmark XT (Ventana, Roche) which can handle up to 30 slides in one run.

FISHversusSISHimagewebcontent

cMET FISH staining on two cell lines using FISH (left) and SISH (right) methods