MET Status (Lung Cancer)

Methodology

To determine the MET status in non-small-cell lung cancer (NSCLC), a validated MET FISH test is used.

Test description:

MET FISH testing: Fluorescence in situ hybridization using the MET (7q31) / SE7 FISH Probe Kit (Kreatech, RUO).

Clinical Implication

Preclinical and clinical evidence suggests a role for MET activation as both a primary oncogenic driver in subsets of lung cancer and as a secondary driver of acquired resistance to targeted therapy. MET gene amplification is associated with acquired resistance to EGFR inhibitors and several monoclonal antibodies and small-molecule inhibitors of MET have been evaluated in clinical trials 1,2,3. In patients with advanced non-small-cell lung cancer, small-molecule inhibitors of MET, showed clear efficacy with a generally tolerable adverse events profile 1.

Specimen Requirements

Acceptable specimens for the assay are formalin (10% buffered formalin)-fixed (6-48 hours), paraffin-embedded lung tissue specimens. Sectioned slides must be analyzed within 24 months after sectioning date.

Volume

1 representative paraffin block is preferred. Alternatively, 3 unstained freshly sectioned tissue sections of 4 µm thickness on positively charged slides are accepted (1 slide for H&E staining, 2 slides for MET FISH testing).

Storage and Shipment Instructions

Maintain and ship specimens at ambient temperature.

Limitations

Fixatives other than buffered formalin, prolonged fixation time, decalcification or exposure to severe heath or chemicals may give rise to inadequate results.

Special Requirements

None

Turn-Around Time

Five to 7 business days for slides and paraffin blocks respectively.

References

  1. Mo and Liu. Targeting MET in cancer therapy. Chr disease and Transl Medicine 3. 2017
  2. Drilon et al. Targeting MET in Lung Cancer: Will Expectations Finally Be MET? J Thor Onco 12. 2016
  3. Kubo et al. MET gene amplification or EGFR mutation activate MET in lung cancers untreated with EGFR tyrosine kinase inhibitors. Int J Cancer 124. 2009.

Updated on 09 April 2019